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CanFel’s development platform is shown schematically. We begin with the generation of proprietary canine and feline tumor cell lines using a technique designed to maintain their in-vivo function. Panels of antibodies are generated by immunizing mice with these cells. This causes the immune system of the mouse to create antibodies specifically targeting naturally expressed dog or cat cell surface proteins. This approach has been shown to generate large numbers of highly specific and functionally interesting antibodies to cancer targets.
Antibodies generated are then evaluated and prioritized using three criteria:

    1. Specific binding to cancers compared to normal tissue.
    2. Assessment of in vitro activity against cultured cat or dog cells.
    3. Assessment of in vivo activity in animal xenograft models using CanFel tumor-derived cell lines.

Antibodies are selected for molecular engineering to make them compatible with the dog or cat immune system and then produced using recombinant protein technology for the initiation of clinical trials and ultimate approval as drugs. Antibodies are selected for development based on the following criteria of the chosen target:

    1. They are validated by proven activity against human cancers
    2. They are present in canine or feline cancer
    3. They are commercially viable
    4. They provide a benefit over the current standard of care
For more, see below ↓
In Vitro Tumor Biology
Feline gastric carcinoma cells, 40x40x
Feline gastric carcinoma cells, 100x100x
Grown in a proprietary medium, in an appropriate microenvironment, we can isolate and establish, in the laboratory, cell lines which are physiologically relevant, as they can mimic the tissue of origin. Years of expertise and experience have combined to create an environment in the laboratory setting, which allows us to study the subtle dynamics of tumor progression, outside of the organism.
A single cell has within it, all the information and complexity common to the tumor, in vivo, that allows us to examine the molecular mechanisms and growth kinetics of the tumor, in vitro. It becomes a powerful tool in the field of tumor biology.The images seen here are examples of feline gastric carcinoma cells, isolated and established as a cell line in culture. These can be used both as immunogens for deriving antibodies against this type of cancer, and as tools to study tumor progression.
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